Hauptcharaktere: Professor Hershel Layton - Luke Triton - Emmy Altava - Jean Descole - Inspektor Clamp Grosky - Kommissar Leonard Bloom - Maskierter Gentleman/Randall Ascot - Henry Ledore - Angela Ledore - Alphonse Dalston - Hauptkommissar Sheffield
Roland Layton - Lucille Layton - Mysterella - Rhys Williams - Lady Ascot - Lord Ascot - Pascal - Jean-Paul - Ludmilla - Gustav - Gonzales - Drake - Confetti - Waltham - Mordaunt - Pirouette - Jojo - Puck - Rossi - Murphy - Bonnie - Tanya - Nordmann - Serena - Palloni - Beaufort - Michelle - Nils - Frankie - Narcisse - Humbert - Aldus - Hannah - Polizist - Yuming - Gloria - Lionel - Dougal - Collins - Leon Bronev - Robotermumie