Hauptcharaktere: Professor Hershel Layton - Luke Triton - Flora Reinhold - Don Paolo - Inspektor Chelmey - Barton - Dr. Dimitri Allen/Dr. Alain Stolypin/Layton der Zukunft/Barkeeper - Clive Dove/Älterer Luke - Claire Folley/Celeste Folley - Bill Hawks
Hora - Tickley - Schrader der Zukunft - Delmona der Zukunft - Dekan Delmona - Shipley - Oma Enygma - Lola Enygma - Bostro - Gangster - Snapper - Frankston - Marzano - Layman - Fisheye - Abelin - Chelton - Barmey - Argus - Smith - Florence - Vito - Art - Nicolas - Anita - Alfie - Pennick - Adeline - Max - Becky - Margaret - Paillard - Dupree - Natalia - Harold - Horace - Hardy - Cuthbert - Segal - Rosalind Stone - Colby - Rudolph - Misha - Dylan - Vivian - Chili - Gammon - Dr. Avogadro - Maya - Maggie - Belle - Graham - Leo - Arnie - Berta Avogadro - Minnie - Novella - Raleigh - Lightie - Mark - Schnurrbert - Polo - Caroline Hawks - Ansager - Constance Dove - Clives Eltern